The Loxahatchee River Preservation Initiative is the outgrowth of a watershed management effort that the Florida Department of Environmental Protection spearheaded in 1996. This multi-agency and stakeholder-based advisory group was organized primarily for the purpose of soliciting, ranking, and submitting to the Florida Legislature a list of projects focused on the preservation and restoration of the water quality and habitats of the Loxahatchee River and its watershed. Agencies and stakeholders are given an avenue to apply for funding on several key projects that are critical to preserving the long-term health of the Loxahatchee River and have not been implemented due to lack of resources and other regional priorities taking precedence.

The District participates as a member of the Loxahatchee River Preservation Initiative due to its location within the Loxahatchee River watershed. The District applied for grant funding in the amount of $75,000 for the Loxahatchee River Headwaters Easement Mapping in Jupiter Farms project, with $75,000 in proposed matching funds by the District. The project was ranked second by the Loxahatchee River Preservation Initiative on August 5, 2024, for a grant funding request for the upcoming legislative session.